Table 2.

TCR53 p:MHC ligand prevalence among tumor and normal cells other than renal cell carcinoma or normal kidney cells

Other tumor cells (origin)HLA-A2HLA-A2 transfectionB3Z-TCR53 IL-2 (pg/mL)
624.38(1)+≤12
93.04-A12(1)+≤12
A-375(1)+≤12
A-673(2)+≤12
BLM(1)+56
BOE(3)≤12
BOE(3)+≤12
CCL-121(2)≤12
COLO-205(4)+≤12
COLO-357(5)≤12
CRL-1543(2)+≤12
CRL-1544(2)+≤12
D-283(6)≤12
D-458(6)≤12
DU-145(7)+≤12
EWING-AK(2)≤12
FADU(8)≤12
FADU(8)+≤12
GRANTA-519(3)+350
HBL-2(3)+≤12
HT-29(4)+≤12
HTC-116(4)+≤12
JEKO-1(3)+≤12
JVM-2(3)+≤12
K-562(9)≤12
K-562(9)+≤12
KARPAS-422(3)+≤12
KELLY(10)≤12
L-428(3)≤12
LCL-1(11)+57
LCL-2(11)+≤12
LCL-3(11)+≤12
LCL-4(11)+25
LCL-26(11)+≤12
LNCAP(7)+≤12
MACA-1(12)≤12
MACA-1(12)+≤12
MCF-7(12)+≤12
MG-63(2)≤12
NALM-6(3)+49
PANC-TU1(5)+136
PC-3(7)+≤12
PCI-1(8)+≤12
SAO-S2(2)+≤12
SK-23(1)+≤12
SK-MEL25(1)+≤12
SK-MEL29(1)≤12
SK-MEL29(1)+≤12
SK-NSH(10)+39
SKW-6(3)+35
SW-480(4)+≤12
SW-620(4)+≤12
TC-71(2)+≤12
THP-1(9)+13
U2-OS(2)+≤12
U-87(13)+≤12
U-251MG(14)+45
U-373(13)+155
UT-SCC-15(8)+313
WM-115(1)+≤12
WM-226.4A(1)+≤12
Other normal cells (origin)
BMEC(15)+≤12
PBMC1-7*(16)+≤12

NOTE: 3Z-TCR53 were cocultured with indicated cells and IL-2 values are listed (12 pg/mL was the background value of B3Z-TCR53 cells alone). Cell lines were collected through laboratory exchanges. HLA-A2 transfection was done using RNA. HLA-A2 status was determined by flow cytometry. The numbers in parenthesis after the cell name indicate the histologic origin: (1) melanoma, (2) sarcoma, (3) B-lymphocytic leukemia/lymphoma, (4) colon carcinoma, (5) pancreas carcinoma, (6) medullablastoma, (7) prostate carcinoma, (8) squamous carcinoma, (9) myelocytic leukemia, (10) neuroblastoma, (11) EBV-B-lymphoblastoid cell line, (12) breast carcinoma, (13) glioblastoma, (14) astrocytoma, (15) brain microvascular endothelial cells, and (16) peripheral blood mononuclear cells (PBMC).

  • *1-7 indicates PBMC of seven different donors.