Table 1.

Primers used for RT-PCR analysis

ApplicationGene nameSequenceTm
IFN-γ signaling JAK1fw: 5′-gca cca tca ccg ttg atg ac-3′60°C
rev: 3′-tcc agt gag ctg gca tca ag-5′
JAK2fw: 5′-tgt gga gat gtg ccg gta tg-3′60°C
rev: 3′-att acg ccg acc agc act gt-5′
STAT1fw: 5′-atc ctc gag agc tgt cta-3′55°C
rev: 3′-gcc agg tac tgt ctg att-5′
Antigen processing LMP2fw: 5′-tgt gca ctc tct ggt tca gc-3′60°C
rev: 3′-tgc tgc atc cac ata acc at-5′
LMP10fw: 5′-ggg ctt ctc ctt cga gaa ct-3′60°C
rev: 3′-cag ccc cac agc agt aga tt-5′
TAP1fw: 5′-gga atc tct ggc aaa gtc ca-3′60°C
rev: 3′-tgg gtg aac tgc atc tgg ta-5′
TAP2fw: 5′-cca aga cgt ctc ctt tgc at-3′60°C
rev: 3′-ttc atc cag cag cac ctg tc-5′
Tapasinfw: 5′-tgg gta agg gac atc tgc tc-3′60°C
rev: 3′-acc tgt cct tgc agg tat gg-5′
HLA-ABCfw: 5′-gcc tac gac ggc aag gat tac-3′60°C
rev: 3′-ggt ggc ctc atg gtc aga ga-5′
Locus specificity RFX3fw: 5′-aaa ctg gac cca gtc aat gc-3′57°C
rev: 3′-tgt tgc atg ggt tgt tgt ct-5′
RCL1fw: 5′-tct tct ttg ctt ggc tcc at-3′57°C
rev: 3′-atg ggc ttc aag acc ttc ct-5′
GLDCfw: 5′-tcg atg cag ttc acc tca ag-3′57°C
rev: 3′-cag att ccc acc tga gca tt-5′
BNCfw: 5′-gtc aag cat gcc tgt gaa ga-3′59°C
rev: 3′-ggc cct cct tat ttc agt cc-5′
Control GAPDHfw: 5′-cct gca cca cca act gct ta-3′60°C
rev: 3′-ctg aag gcc atg caa gtg ag-5′
PPIAfw: 5′-cca tct atg ggg aga aat ttg a-3′60°C
rev: 3′-cag tca gca atg gtg atc ttc t-5′
β-Actinfw: 5′-tcc tgt ggc atc cac gaa act-3′60°C
rev: 3′-gaa gca ttt gcg gtg gac gat-5′

Abbreviations: fw, forward; rev, reverse.