Table 3

Percentage of apoptosis after treatment of NCI-H661 cells with flavopiridol

Prolonged exposure produces a greater apoptotic response in a representative experiment in NCI-H661 cells. Cells were treated with DMSO or the indicated doses.
24/24a24/72b72/72c72/120d120/120e
DMSO<1<1<1<110
300 nm15<1185758
400 nm215476791
500 nm2416738892
1000 nm312680NDfND
1500 nm463571NDND
2000 nm392158NDND
  • a 24/24, cells were treated for 24 h and collected at 24 h.

  • b 24/72, cells were treated for 24 h and collected at 72 h.

  • c 72/72, cells were treated for 72 h and collected at 72 h.

  • d 72/120, cells were treated for 72 h and collected at 120 h.

  • e 120/120, cells were treated for 120 h and collected at 120 h. The percentage of apoptosis was determined by TUNEL assay. At all doses, there is more apoptosis after continuous treatment for 72 h, compared with treatment only for 24 h. In addition, flavopiridol-mediated cytotoxicity is not reversed when drug is removed at 72 h.

  • f ND, not determined.