Table 2

Patient characteristics and experimental results

Ascites fluid was collected from 27 patients with stage III or IV ovarian cancer. All 27 cases contained cells that bound cytokeratin. Assays were prioritized, depending upon the number of cells obtained. Binding of MIS-biotin was always to the cytokeratin-positive epithelial tumor population; cells from 15 of 27 patients (56%) exhibited MIS-biotin binding (+). RT-PCR was performed only if cells could be studied immediately; hence, the number of patients available for this analysis is smaller. Of the 15 patients who exhibited binding, 9 were analyzed for exons 2–6, which include the extracellular domain of the MIS type II receptor mRNA, and 8 of 9 expressed the message (+). The two patients that did not bind MIS but were positive by RT-PCR may reflect the sensitivity of PCR-based assays. The ascites cells of 11 of the patients who bound MIS were used in antiproliferation assays. Inhibition of colony growth was seen in 82% of these patients.
Patient no. (not sequential)Binding of MIS-biotin to ascites cells (+/−)MIS type II receptor message in ascites cells (+/−)Colony inhibition of ascites cells
% inhibitionP
1+NAa49%<0.05
2+NA49%<0.05
3++65%<0.05
4+NANo growthb*c
5++29%<0.05
6+NA0%NSd
7++NA*
8++13%NS
9++41%<0.001
10++33%<0.012
11+32%<0.013
12+NA33%<0.001
13+NANA*
14++94%<0.021
15++NA*
16No growth*
17NANo growth*
18NANo growth*
19No growth*
20+No growth*
2129%NS
22NANo growth*
23NANA*
24+34%<0.001
2551%<0.001
26NA34%<0.019
27NANA*
  • a NA, insufficient cells remained for additional study, or in the case of RT-PCR analysis, that the sample was received >4 h after collection.

  • b Cells did not form colonies in soft agar and, therefore, could not be evaluated.

  • c *, statistical tests not performed because cells did not grow.

  • d NS, not significant.