Table 1

Screening of a panel of 18 human tumor cell lines for SarCNU cytotoxicity and ERCC2, MGMT, and EMT gene expression

Cell line (tumor type)SarCNU cytotoxicity IC90m)Protein levelmRNA level EMTd
MeanSEERCC2bMGMTcMeanSE
MeanSEMeanSE
MCF (B)a218.513.50.0070.0041.00000.3840.066
SKMG-1 (G)28.62.70.0040.002000.7400.085
T98G (G)165.21.40.0570.0260.3060.1270.2270.138
SKMG-4 (G)40.62.30.0610.0220.0520.0250.5960.058
HT-29 (C)80.75.60.0030.0030.6130.0890.8830.097
SKI-1 (G)44.81.20.0690.0290.0120.0120.0530.029
SF-295 (G)46.50.80.0660.0290.0370.0370.1850.057
MGR-3 (G)266.413.50.0370.0150.0220.0220.5200.080
MGR-2 (G)36.82.30.0280.012000.1350.042
786-0 (R)36.72.30.0830.0540.0230.0190.2290.071
CAKI-1 (R)284.829.00.1090.0440.3940.1390.8230.117
ACHN (R)146.211.60.2080.0880.6800.0110.5220.095
UW28 (G)62.27.60.0290.0120.1340.1340.3740.096
A498 (R)451.614.40.3260.0990.1300.0831.1160.064
MGR-1 (G)72.43.50.0090.003000.0700.026
SKNSH (N)367.742.71.00000.1670.0940.1000.023
SW-620 (C)34.05.30.0060.0040.0150.0060.6700.047
UWR-7 (G)33.42.70.1840.067000.3770.091
  • a Tumor types of cell line: B, breast; C, colon; G, glioma; N, neuroblastoma; R, renal.

  • b ERCC2 protein level as determined by Western blot analysis for each cell line was divided by ERCC2 protein level for SKNSH for each experiment. The result is expressed as the mean value of at least three separate experiments.

  • c MGMT protein level determined by Western blot analysis for each cell line was divided by MGMT protein level for MCF-7 for each experiment. The result is expressed as the mean value of at least three separate experiments.

  • d EMT mRNA level determined by RT-PCR for each cell line was divided by mRNA level for HepG2, which is used as positive control for each experiment. The result is the mean value of three repeated experiments.