Table 3

Immunochemical analysis of microvessel density and VEGF, IL-8, and bFGF protein production after therapy with MAb C225

Microvessel countVEGFaIL-8abFGFa
Controlb185 ± 30259 ± 20155 ± 2990 ± 10
MAb C225
 Week 1200 ± 16235 ± 6283 ± 576 ± 6c
 Week 3193 ± 15174 ± 3021 ± 3d33 ± 14c
 Week 584 ± 4d56 ± 15d34 ± 1d14 ± 9c
  • a VEGF, IL-8, and bFGF cytoplasmic staining was evaluated by computer-assisted image analysis and is expressed as a ratio of tumor expression to normal urothelium expression.

  • b Control mice were sacrificed when they became moribund about 4–6 weeks after tumor implantation. No significant difference in microvessel density or VEGF, IL-8, and bFGF expression was observed in control mice sacrificed between 4 and 6 weeks.

  • c P < 0.05.

  • d P < 0.005.