Table 1

Colony-forming ability in soft agar of HPCA-10a clones

IL-10TGF-β1
CloneVehicle10 ng/ml30 ng/ml1 ng/ml10 ng/ml
HPCA-10a244 ± 20255 ± 17380 ± 33291 ± 18256 ± 20
P < 0.001P < 0.05
HPCA-10a-TGF-β1339 ± 26384 ± 29244 ± 26389 ± 20444 ± 30
P < 0.001
HPCA-10a-IL-10222 ± 16284 ± 19214 ± 11282 ± 23334 ± 20
P < 0.05P< 0.05P < 0.001
HPCA-10a-TGF-β1-IL-10a230 ± 22252 ± 30280 ± 19301 ± 21350 ± 40
P < 0.05P < 0.001
  • a HPCA-10a-TGF-β1 cells were cotransfected with the IL-10 gene. All experiments were carried out in medium containing 2% serum. Each data point represents the total number of colonies counted per 12-mm dish after plating with 1 × 104 cells for 72 h. Each data point represents the mean ± 1 SD from three dishes/experiment and three experiments.