Table 2

Comparison of ploidy, immunohistochemistry, and LOH frequencies among 12 lung tumor cell lines and their corresponding tumor tissues

Comparison of tumor tissue/cell line
FeatureFrequency Tumor tissue/Cell line+/++/−−/+−/−Concordance
Protein expressiona
 HER2/neu25%/25%300912/12 (100%)
 p53 protein100%/100%1200012/12 (100%)
LOH frequencies by chromosomal regionsb
 3p2538%/38%30058/8 (100%)
 3p22–2455%/55%600511/11 (100%)
 3p2158%/58%700512/12 (100%)
 3p14–2122%/22%20079/9 (100%)
 3p14.2 (FHIT gene)50%/50%500510/10 (100%)
 3p1225%/25%20068/8 (100%)
 Any 3p67%/67%800412/12 (100%)
 5q22 (APC-MCC region)44%/44%40059/9 (100%)
 8p2391%/91%1000111/11 (100%)
 8p2283%/92%1001111/12 (92%)
 8p2158%/75%702310/12 (83%)
 Any 8p100%/100%1200012/12 (100%)
 9p2178%/89%70118/9 (89%)
 13q (RB gene)33%/33%20046/6 (100%)
 17p (TP53 gene)78%/89%70118/9 (89%)
 Total920553145/150 (97%)
MA42%/58%60249/12 (75%)
Gene mutations
TP53 gene (exons 5–8)50%/83%60428/12 (67%)
 K-ras gene (codons 12–13)17%/17%200012/12 (100%)
  • a As determined by immunostaining.

  • b For LOH studies: +, LOH; −, retention of heterozygosity.