Table 2

Effects of WHI-P131 against clonogenic leukemic cells

Experiment no.WHI-P131 concentration(μm)Cell linesa
Mean no. of colonies/105 cells% inhibitionMean no. of colonies/105 cells% inhibition
NALM-6 (pre B-ALL)BT20 (breast cancer)
102890 (2660, 3120)NA2676 (2712, 2640)NA
0.12970 (2756, 3184)0NDND
13180 (3080, 3136)0NDND
101932 (1864, 2000)33.23298 (2940, 3656)0
1002 (2, 2)>99.92190 (1632, 2748)18.2
DAUDI (B-ALL)M24-MET (melanoma)
203950 (3100, 4800)NA177 (120, 235)NA
0.32030 (1700, 2360)48.6312 (238, 386)0
12136 (1568, 2704)45.9287 (157, 418)0
31406 (988, 1824)64.4390 (280, 500)0
101149 (1054, 1244)70.9301 (249, 353)0
3029 (12, 46)98.0599 (534, 664)0
RAMOS (B-ALL)SQ20B (squamous carcinoma)
301286 (1164, 1408)NA754 (452, 1056NA
302 (0, 4)99.8838 (600, 1076)0
MOLT-3 (T-ALL)HL-60 (AML)
01322 (1252, 1392)NA1854 (1648, 2060)NA
1001 (1, 1)>99.91 (1, 1)>99.9
  • a Cells were treated with WHI-P131 and then assayed for colony formation, as described in “Materials and Methods.” NA, not applicable; ND, not determined. %T-ALL, T-cell ALL; AML, acute myelageneous leukemia.