Table 2

Expression of dominant-negative and mutant Ikaros isoforms in leukemic cells from children with T-lineage ALLa

Ikaros expression profile
PatientIntracellular localization (confocal microscopy)Provisional isoform assignment by Western blot analysisRT-PCR products
CytoplasmNucleusIk-1 (Mr 57,000)Ik-2/3 (Mr 47,000)Ik-4 → Ik-8 (Mr 37,000–40,000)Apparent size (kb)Identity by sequence analysis
T-ALL#1++++++NDND
T-ALL#2++++++1.1Ik-4(WT)
T-ALL#3+++(D)++++1.1Ik-4(del)
T-ALL#4ND++1.1Ik-2(del), Ik-7(del)
T-ALL#5ND+++++1.2/1.0Ik-2(WT), Ik-4(WT)
T-ALL#6++++++1.2/1.0Ik-2(del), Ik-4(WT)
T-ALL#7ND+++++NDND
T-ALL#8ND+++++++NDND
T-ALL#9ND+NDND
T-ALL#10+++++++NDND
T-ALL#11++++++++1.2/1.1/1.0Ik-2(WT), Ik-4(WT)
Ik-4(del), Ik-8(del)
T-ALL#12++++(D)+++1.0Ik-2(del), Ik-4(del)
T-ALL#13ND+++NDND
T-ALL#14+++(D)ND1.2Ik-2(ins), Ik-2(ins,del)
T-ALL#15+++(D)+++++NDND
T-ALL#16++++++1.0Ik-4(WT)
T-ALL#17ND+++++NDND
T-ALL#18++++++(D)+++++1.2/1.1/1.0Ik-1(WT), Ik-2(WT)
Ik-4(WT), Ik-4(del)
MOLT-3 Cell line++++++++1.2/0.9Ik-2(ins,del), Ik-8, Ik-8(del)
JK-E6-1 Cell line++++++++1.1Ik-2, Ik-4(WT)
FT+++(P)+++1.4Ik-1(WT)
NTHY-1+++(P)++++++NDND
NTHY-2+++(P)++NDND
NTHY-3+++(P)++++++NDND
NBM-1+++(P)++++++1.2Ik-2(WT)
NBM-2+++(P)++NDND
  • a Confocal microscopy, Western blot analysis, and RT-PCR analysis including PCR cloning and sequencing were performed as described in “Materials and Methods.” +++, strongly positive; +, weakly positive; −, negative; ND, not determined; WT, wild-type; del, deletion mutant. Numbers in parentheses indicate the number of PCR clones sequenced. The provisional assignment of Ikaros isoform designations by Western Blot analysis was based upon the size of the immunoreactive Ikaros proteins in whole-cell lysates. NTHY-1, 2, 3, normal thymocytes from children without leukemia; NMB-1, 2, normal bone marrow mononuclear cells from children without leukemia; FT, fetal thymocytes; D, diffuse staining; P, punctate staining.