Cell cycle phase distribution of HT29 cells after TAX chronic exposure
Cells were treated with various TAX concentrations and subjected to flow cytometric analysis after treatment as described in “Materials and Methods.” Culture medium with TAX was changed after 48 h. | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Treatment | % | Time (h) | ||||||||||
24 | 48 | 72 | 96 | 120 | ||||||||
G1 | 55a | 66 | 75 | 66 | 72 | |||||||
Control | S | 41 | 22 | 18 | 30 | 22 | ||||||
G2/M | 4 | 12 | 7 | 4 | 6 | |||||||
G1 | 53 | 66 | 75 | 67 | 71 | |||||||
0.5 nm | S | 42 | 21 | 19 | 27 | 21 | ||||||
G2/M | 5 | 13 | 6 | 6 | 8 | |||||||
G1 | 48 | 64 | 62 | 65 | 70 | |||||||
1 nm | S | 44 | 23 | 25 | 28 | 24 | ||||||
G2/M | 8 | 13 | 13 | 7 | 6 | |||||||
G1 | 44 | 63 | 63 | 63 | 70 | |||||||
5 nm | S | 40 | 22 | 24 | 27 | 20 | ||||||
G2/M | 16 | 15 | 13 | 10 | 10 | |||||||
G1 | 48 | 63 | 69 | 73 | 71 | |||||||
10 nm | S | 36 | 22 | 16 | 21 | 21 | ||||||
G2/M | 16 | 15 | 15 | 6 | 8 | |||||||
G1 | 52 | 34 | Fb | 52 | F | |||||||
50 nm | S | 33 | 52 | F | 34 | F | ||||||
G2/M | 15 | 14 | 15 | 14 | 15 | |||||||
G1 | 1 | 11 | F | F | F | |||||||
100 nm | S | 4 | 19 | F | F | F | ||||||
G2/M | 95 | 70 | 88 | 90 | 80 |
a Data represent mean values from three separate experiments; SD, <5%.
b F, cells or nuclei detected as damaged.