Cell cycle phase distribution of LoVo cells after TAX chronic exposure
Cells were treated with various TAX concentrations and subjected to flow cytometric analysis after treatment as described in “Materials and Methods.” Culture medium with TAX was changed after 48 h. | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Treatment | % | Time (h) | ||||||||||
24 | 48 | 72 | 96 | 120 | ||||||||
G1 | 41a | 59 | 51 | 63 | 68 | |||||||
Control | S | 34 | 16 | 29 | 20 | 17 | ||||||
G2/M | 25 | 25 | 20 | 17 | 15 | |||||||
G1 | 40 | 58 | 56 | 62 | 66 | |||||||
1 nm | S | 35 | 17 | 24 | 19 | 18 | ||||||
G2/M | 25 | 25 | 20 | 19 | 16 | |||||||
G1 | 40 | 55 | 55 | 61 | 66 | |||||||
5 nm | S | 35 | 19 | 26 | 17 | 15 | ||||||
G2/M | 25 | 26 | 19 | 22 | 19 | |||||||
G1 | 40 | 51 | 56 | 58 | 68 | |||||||
10 nm | S | 34 | 22 | 30 | 20 | 12 | ||||||
G2/M | 26 | 27 | 14 | 22 | 20 | |||||||
G1 | 38 | 49 | 51 | 53 | 65 | |||||||
50 nm | S | 34 | 20 | 21 | 22 | 12 | ||||||
G2/M | 28 | 31 | 28 | 25 | 23 | |||||||
G1 | 37 | 44 | 55 | 44 | 52 | |||||||
100 nm | S | 30 | 21 | 23 | 25 | 20 | ||||||
G2/M | 33 | 35 | 22 | 31 | 28 | |||||||
G1 | 21 | 18 | 10 | 9 | 9 | |||||||
1,000 nm | S | 19 | 16 | 35 | 35 | 36 | ||||||
G2/M | 60 | 66 | 55 | 56 | 55 | |||||||
G1 | 9 | 14 | 9 | 11 | 10 | |||||||
10,000 nm | S | 8 | 15 | 11 | 8 | 5 | ||||||
G2/M | 83 | 71 | 80 | 81 | 85 |
a Data represent mean values from three separate experiments; SD, <5%.