Table 3

Efficacy of the combination of rMETase and CDDP on growth of human colon tumors in nude mice

Human colon tumors Colo 205 and SW 620 were transplanted s.c. bilaterally to nude mice. Five days after transplantation, the mice were randomized to six per group. The mice were treated with rMETase (5 units/g every 8 h, i.p., days 5–9) and CDDP (7 mg/kg, once on day-5 or day-10, i.p.). Tumor size and body weight were measured every 3 days until day-16, which was 7 days after the last treatment. The T:C value and P (Student’s t test) were then calculated from these data.
GroupAgentTreatmentResults (day-12)P (comparison to control)
RouteDoseScheduleaT:C (%)BW (%)
A. Efficacy of the combination of rMETase and CDDP on growth of human colon tumor Colo 205 in nude mice
ABufferi.p. q 8 h0.5 mlDays 5–9100120
BrMETasei.p. q 8 h5 units/gDays 5–940113<0.05
CCDDPi.p. once7 mg/kgDay-544120<0.05
DCDDPi.p. once7 mg/kgDay-1042105<0.05
ErMETase +i.p. q 8 h5 units/gDays 5–9
CDDPi.p. once7 mg/kgDay-58104<0.001
FrMETase +i.p. q 8 h5 units/gDays 5–9
CDDPi.p. once7 mg/kgDay-1019110<0.01
B. Efficacy of the combination of rMETase and CDDP on growth of human olon tumor SW 620 in nude mice
ABufferi.p. q 8 h0.5 mlDays 5–9100105
BrMETasei.p. q 8 h5 units/gDays 5–95698<0.05
CCDDPi.p. once7 mg/kgDay-580110>0.05
DCDDPi.p. once7 mg/kgDay-1072115>0.05
ErMETase +i.p. q 8 h5 units/gDays 5–9
CDDPi.p. once7 mg/kgDay-52596<0.01
FrMETase +i.p. q 8 h5 units/gDays 5–9
CDDPi.p. once7 mg/kgDay-104780<0.05
  • a Day-0 was the day of tumor transplantation.