Table 1

Phenotypic characterization of SKOv-3 c-src transfectants

Cell lineDoubling time (h/days)aAdherence to plasticbAdherence to collagen IVSaturation densityc
SKOv-3 (parental cells)32.2/1.3445,000 ± 2,600NODd0.606 ± 0.074
S20 (sense)36.4/1.5236,000 ± 3,500NOD0.700 ± 0.061
AS16 (antisense)34.4/1.4337,000 ± 1,600NOD0.750 ± 0.060
2AS4 (antisense)26.3/1.1043,000 ± 2,900NOD0.726 ± 0.103
  • a Doubling time was calculated using the formula Dt = (0.69)(t)/1n Mf − 1n Mi, where t is time in hours between plating and counting, Mf is the cell number at time t, and Mi is the cell number at time 0.

  • b Adherence assays were performed as described in “Materials and Methods.” Values shown represent mean fluorescence ± SD, with four replicates/sample. Values shown are in growth medium containing 10% FCS. Similar values were obtained in 0.2% FCS.

  • c Saturation density assay was performed as described in “Materials and Methods.” Values shown represent mean absorbance ± SD, with eight replicates/sample.

  • d NOD, no observable difference between sample groups.