Table 1

PCR primers for determination of intron-exon boundaries of the DPD gene

ExonGene-specific primer 1Gene-specific primer 2
4a5′-ATGCCTGAAATGTGCAGATGCC-3′5′-CAGAAGAGCTGTCCAACTAATCTTG-3′
9a5′-GATGCCATCTTCCAAGGCCTG-3′5′-GCAGGACCAGGGGTTTTATACATC-3′
12a5′-TGTGAATGTACCAAGAAGCTTGCT-3′5′-CTGCAAATACCCATGCTTCACTAGT-3′
12a5′-CAGAAGTAGATCCAGAAACTATGC-3′5′-CATGGGTATTTGCAGGTGGTGATG-3′
15b5′-CTCAGACTTCTTGGCAAGTTCC-3′5′-GTCCAGTCATTTTTATTGTAACTGC-3′
21a5′-GCCAAGTTTTGGACCTTATCTGG-3′5′-AATGTAGCTTTTCACCACTTAAGAG-3′
  • a For determination of the 5′ donor site.

  • b For determination of the 3′ acceptor site.