Table 2

Oligonucleotides used for genomic PCR of DPD sequences

ExonPrimersPrimer sequencesPCR temperature (°C)Fragment (bp)
1DPDEIF5′-GCTGTCACTTGGCTCTCT-3′50184
DPD111R15′-CACCTACCCGCAGAGCA-3′
2M13DPD108F5′-M13F-GTGACAAAGTGAGAGAGACCGTGTC-3′a65321
M13DPD109R5′-M13R-GCCTTACAATGTGTGGAGTGAGG-3′b
3DPD112F5′-GAATGCTACCCAATTAAAGTGG-3′55267
DPD113Rd5′-CCTACCACCATCCTGTGACTG-3′
4DPD96F5′-GGTAGAAAATAGATTATCTC-3′50245
DPD4R5′-GATTTGCTAAGACAAGCTG-3′
5DPD94F5′-GTTTGTCGTAATTTGGCTG-3′60284
DPD95R5′-ATTTGTGCATGGTGATGG-3′
6DPD92F5′-GAGGATGTAAGCTAGTTTC-3′52357
DPD93R15′-CCATTTGTGTGCGTGAAGTTC-3′a
7DPD90F5′-GTCCTCATGCATATCTTGTGTG-3′b52360
DPD121R5′-GCTTCTGCCTGATGTAGC-3′
8DPD122F5′-GCCCCACATCGTGCTATGAACA-3′57459
DPD91R15′-GTCTGAAGGCAGTCATTCTGG-3′
9M13DPD88F5′-M13F-CCCTCCTCCTGCTAATG-3′a52278
M13DPD9R-25′-M13R-GAACAATGTGCTGCTGAG-3′b
10DPD86F5′-GAGAGTGACACTTCATCCTGG-3′60342
DPD87R5′-CTGTTGGTGTACAACTCC-3′
11DPD845′-ACTGGTAACTGAAACTCAG-3′52442
DPD85R45′-CAATTCCCTGAAAGCTAG-3′
12DPD12F5′-TTCCTGTATGTGAGGTGTA-3′55453
DPD83R15′-GAAGCACTTATCCATTGG-3′
13DPD70F5′-CGGATGACTGTGTTGAAGTG-3′55440
DPD71R5′-TGTGTAATGATAGGTCGTGTC-3′
14DPD14F5′-TCCTCTGCAAAAATGTGAGAAGGGACC-3′55370
DPD14R5′-TCACCAACTTATGCCAATTCTC-3′
15M13DPD15F5′-M13F-TATCTTTGTGTACAACTGGA-3′a52391
M13DPD119R5′-M13R-TGTGAAATCCAAGGGACC-3′b
16DPD116F5′-AACGGTGAAAGCCTATTGG-3′52223
DPD117R5′-TAGTAACTATCCATACGGGGG-3′
17DPD114F5′-CACGTCTCCAGCTTTGCTGTTG-3′60238
DPD115R5′-CGGGCAACTGATTCAAGTCAAG-3′
18DPD98F5′-TGGGATGTGAGGGGGTGAATG-3′60248
DPD99R5′-TTCAGCAACCTCCAAGAAAGCCAC-3′
19DPD100F5′-TGTCCAGTGACGCTGTCATCAC-3′55300
DPD80R5′-CATTGCATTTGTGAGATGGAG-3′
20DPD78F5′-GAGAAGTGAATTTGTTTGGAG-3′55424
DPD79R15′-CACAGACCCATCATATGGCTG-3′
21M13DPD76F5′-M13F-TCTGACCTAACATGCTTC-3′a48264
M13DPD21R5′-M13R-CCAGTAAAGTAGGCATAC-3′b
22DPD74F5′-GAGCTTGCTAAGTAATTCAGTGGC-3′60291
DPD75R5′-AGAGCAATATGTGGCACC-3′
23DPDASPF5′-GGGGACAATGATGACCTATGTGG-3′60269
DPDASPR5′-GGTGACATGAAAGTTCACAGCAAC-3′
  • a Sequence M13F, TGT-AAA-ACG-ACG-GCC-AGT.

  • b Sequence M13R, CAG-GAA-ACA-GCT-ATG-ACC.