Table 1

Histological analysis of tumor dissemination in the intrapleural lung cancer models

DayHeartLungMediastinumDiaphragmLiverDuodenumStomachPancreasMesenteric lymph nodes
NCI-H460
 5+/−N+/−+NNNNN
 7+/−+++++NNNNN
11N+++++++NNNNN
14+/−++++++++++/−N+/−+/−N
19+/−+++++++++++N+/−+/−+/−
25+/−++++++++++++/−+++++++
A549
 4N+/−+/−+NNNNN
11+/−++++NNNNN
14+/−++++++NNNNN
21+/−+++++++/−NNNN
31+/−++++++++NNNNN
39N+++++++NNNNN
  • Tumor cells (106) were implanted in the pleural space of nude mice on day 0. Two or three mice were sacrificed on each of the indicated days, and organs were removed and fixed for histopathological characterization. +/−, at least one mouse presenting tumor nodules;+ to +++, all mice presenting tumor nodules, from a few nodules (+) to a massive invasion (+++). N, normal.