Table 2

Effect of C225, radiation, or both on apoptosis and necrosis in A431 tumor xenograftsa

Time after irradiationbNo treatmentC225 treatment18 Gy irradiationC225 + 18 Gy irradiation
Apoptosis (%)Necrosis (%)Apoptosis (%)Necrosis (%)Apoptosis (%)Necrosis (%)Apoptosis (%)Necrosis (%)
0 h0.7 ± 0.4c10.4 ± 2.10.8 ± 0.219.9 ± 6.2
4 h0.7 ± 0.219.7 ± 8.02.2 ± 0.621.7 ± 5.00.9 ± 0.659.8 ± 6.9
1 day1.2 ± 0.413.3 ± 4.61.9 ± 0.141.1 ± 3.51.0 ± 0.457.8 ± 2.0
3 days0.9 ± 0.526.9 ± 10.01.5 ± 0.364.8 ± 8.31.3 ± 0.480.1 ± 6.2
7 days0.5 ± 0.155.5 ± 8.30.2 ± 0.174.8 ± 5.50.3 ± 0.285.9 ± 4.0
  • a Mice bearing 8-mm tumors in the right hind leg were given i.p. 1 mg of C225 antibody, 18 Gy of local tumor irradiation or the antibody plus 18 Gy. When the two agents were combined, C225 was given 6 h before tumor irradiation. Groups consisted of 3–7 mice each.

  • b The times indicated are in relation to the time of local tumor irradiation. In the case of C225 treatment only, 6 h needs to be added to these times. For example, 0 h analysis for apoptosis or necrosis is actually 6 h after administration of C225.

  • c Mean ± SE.