Table 2

Enhanced drug sensitivity in the PDPK FA antisense clone (PA3) of the human prostate carcinoma PC-3 cella

CellsDrugs
Carboplatin5-FluorouracilPaclitaxelHydroxyurea
IC90m)-FoldIC90m)-FoldIC90 (nm)-FoldIC90m)-Fold
PV196.25 ± 7.951.00.62 ± 0.041.022.65 ± 1.501.0710.15 ± 9.071.0
PA35.35 ± 0.6318.00.09 ± 0.016.92.76 ± 0.338.268.38 ± 5.5510.4
  • a The drugs sensitivity in terms of IC90 was determined by clonogenic assay, which was performed by plating control-transfected (PV1) and antisense (PA3) clones (∼500 cells) in a 6-well plate and cultured in different concentrations of drugs as indicated at 37°C for 10 days. The cell colonies were stained by crystal violet. The formed colonies containing> 50 cells were counted. Data were taken from the averages of three independent experiments and expressed as means ± SD. All the values are significant with P < 0.01 (Student’s t test).