Table 2

TS and TP levels and results from immunostaining in patients with colorectal tumors

Enzyme assays and IHS were performed under optimal assay conditions as described in “Patients and Methods.”

A. Enzyme activityRangeMean (SD)Median
TS catalytic activity (pmol/h/mg protein)
1 μm dUMP4.6–118.556 (45)46
10 μm dUMP18.9–1001.8391 (276)192
FdUMP binding (fmol/mg protein)4.8–6926 (12)23
TP activity (nmol/h/mg protein)
Tumor tissue42.9–642201 (126)192
Normal mucosa30.9–246.287 (40)85
B. IHSn%
TS1+18 56
2+14 44
TPPositive14 44
Negative18 56
p53Positive18 56
Negative14 44
Ki67 labeling index≤40% positive cells15 47
>40% positive cells17 53
VEGFPositive22 69
Negative10 31
CD31MVD hotspot ≤40/mm21134
>40/mm221 66
MVD global ≤16/mm217 53
>16/mm215 47