Table 3

mRNA expression of angiogenic factors

Each value was corrected for background and GAPDH expression.

+IRa−IR
+TNP-470−TNP-470+TNP-470−TNP-470
8 h48 h8 h48 h8 h48 h8 h48 h
VEGF0.210.230.180.140.180.260.110.09
(0.11–0.24)(0.20–0.27)(0.09–0.77)(0.04–0.17)(0.05–0.24)(0.07–0.47)(0.03–0.44)(0.03–0.10)
bFGF0.020.030.0100.020.0200
(0–0.03)(0–0.07)(0–0.04)(0–0.03)(0.01–0.05)(0.01–0.03)(0–0.004)(0–0.43)
Ang-1b0.380.65c0.140.260.220.49c0.240.21
(0–0.83)(0.43–1.08)(0.06–0.49)(0.16–0.38)(0–0.40)(0.35–0.72)(0.10–0.75)(0.01–0.58)
Ang-20.771.030.770.650.470.680.770.53
(0.62–1.63)(0.64–1.27)(0.59–1.32)(0.64–1.26)(0.41–1.58)(0.62–1.21)(0.46–1.43)(0.39–1.76)
  • a Median values from 4 membranes; numbers in parentheses, range. Arbitrary units; mRNA from U87 in vitro was loaded on each membrane and the U87 in vitro value was set to 1.

  • b Ang-1, angiopoietin 1; Ang-2, angiopoietin 2.

  • c Significant increased value compared to non-TNP-470 treated tumors.