Table 1

Chemosensitivity to five anticancer agents used in combination with nimesulide

Cell lineSM-5887-13-OHSN-38TaxotereVP-16CDDP
IC50 (μg/ml)RRaIC50 (μg/ml)RRIC50 (μg/ml)RRIC50 (μg/ml)RRIC50 (μg/ml)RR
NSCLC cell lines
ACC-LC-910.015 ± 0.003b1.000.0065 ± 0.00101.000.0032 ± 0.00041.000.21 ± 0.031.002.51 ± 0.421.00
ACC-LC-91/NIM 10c0.008 ± 0.0020.530.0039 ± 0.00080.600.0025 ± 0.00030.780.17 ± 0.020.802.11 ± 0.260.84
ACC-LC-91/NIM 30c0.006 ± 0.0020.400.0015 ± 0.00030.230.0015 ± 0.00020.460.14 ± 0.020.661.90 ± 0.230.75
ACC-LC-3230.025 ± 0.0041.000.0055 ± 0.00081.000.0028 ± 0.00021.000.24 ± 0.021.000.45 ± 0.061.00
ACC-LC-323/NIM 100.023 ± 0.0030.920.0050 ± 0.00070.900.0022 ± 0.00030.780.21 ± 0.030.870.37 ± 0.040.82
ACC-LC-323/NIM 300.021 ± 0.0030.840.0043 ± 0.00060.780.0017 ± 0.00030.600.20 ± 0.020.830.35 ± 0.030.77
ACC-LC-3190.038 ± 0.0051.000.0061 ± 0.00091.000.0060 ± 0.00091.000.50 ± 0.091.002.10 ± 0.331.00
ACC-LC-319/NIM 100.034 ± 0.0040.890.0055 ± 0.00080.900.0051 ± 0.00080.850.45 ± 0.080.902.01 ± 0.280.95
ACC-LC-319/NIM 300.028 ± 0.0040.730.0049 ± 0.00090.800.0042 ± 0.00090.700.43 ± 0.090.861.89 ± 0.240.90
ACC-LC-330.080 ± 0.0101.000.0091 ± 0.00101.000.0082 ± 0.00091.000.82 ± 0.111.002.88 ± 0.381.00
ACC-LC-33/NIM 100.078 ± 0.0090.970.0088 ± 0.00090.960.0073 ± 0.00080.890.80 ± 0.100.972.68 ± 0.350.93
ACC-LC-33/NIM 300.064 ± 0.0090.800.0078 ± 0.00090.850.0070 ± 0.00090.850.73 ± 0.090.892.43 ± 0.400.84
Normal lung epithelial cell lines
BEAS2B0.024 ± 0.0031.000.0061 ± 0.00071.000.0062 ± 0.00061.000.31 ± 0.021.001.31 ± 0.221.00
BEAS2B/NIM 100.023 ± 0.0040.950.0064 ± 0.00051.040.0070 ± 0.00081.120.32 ± 0.031.031.52 ± 0.341.16
BEAS2B/NIM 300.025 ± 0.0021.040.0064 ± 0.00061.040.0064 ± 0.00081.030.33 ± 0.021.061.50 ± 0.261.14
HPL1D0.042 ± 0.0051.000.0110 ± 0.00101.000.0092 ± 0.00091.000.64 ± 0.081.001.73 ± 0.121.00
HPL1D/NIM 100.040 ± 0.0060.950.0099 ± 0.00080.900.0088 ± 0.00110.950.60 ± 0.070.931.71 ± 0.210.98
HPL1D/NIM 300.040 ± 0.0070.950.0098 ± 0.00070.890.0090 ± 0.00100.970.59 ± 0.100.921.92 ± 0.101.10
  • a RR, relative resistance.

  • b Mean ± SD of the results obtained in three independent experiments.

  • c NIM 10 and NIM 30, simultaneous exposure to 10 μm and 30 μm nimesulide, respectively. As a single agent, nimesulide yielded 15% growth inhibition at 10μ m and 28% at 30 μm in ACC-LC-91 cells, 12% and 23% in ACC-LC-323 cells, 8% and 13% in ACC-LC-319 cells, 6% and 10% in ACC-LC-33 cells, 0% and 0% in BEAS2B cells, and 0% and 1% in HPL1D cells (see Fig. 1<$REFLINK> ).