Table 1

Tumorigenicity and production of spontaneous metastases after orthotopic implantation of PC-3P, PC-3P(Neo), and sense IL-8 transfectants and of PC-3M-LN4, PCM-LN4 (AS Neo), and antisense IL-8 transfectant in prostate of nude mice

Cell lineTumorigenicityLymph node metastasis
IncidenceMedian prostate weight (range) (mg)IncidenceMedian lymph node weight (range) (mg)
Sense transfection
PC-3P4 /755 (24–480)4 /720 (16–28)
PC-3P(Neo)6 /9120 (27–443)4 /924 (15–32)
PC-3P(IL-8)8 /8a1,270 (258–1,850)b8 /8a93 (36–124)b
PC-3P(IL-8 Low)5 /8155 (25–720)5 /838 (25–69)
PC-3P(IL-8 High)9 /9a1,975 (355–2,915)b9 /9a131 (55–188)b
Antisense transfection
PC-3M-LN45 /51,921 (250–3,359)5 /5101 (19–155)
PC-3M-LN4(AS Neo)5 /5543 (312–713)5 /5110 (24–122)
PC-3M-LN4(AS IL-8)2 /8c,d(33, 36)d,e0 /8f
PC-3M-LN4(AS IL-8 Low)2 /8c,d(31, 39)d,e0 /8f
PC-3M-LN4(AS IL-8 High)6 /939 (30–525)f0 /9f
  • a P < 0.05 against PC-3P and PC-3P Neo (χ2 test).

  • b P < 0.005 against PC-3P and PC-3P(Neo) (Mann-Whitney statistical comparison).

  • c P < 0.05.

  • d Explants grown in culture; no detectable tumor by immunohistochemistry.

  • e P < 0.0005 against PC-3M-LN4 and PC-3M-LN4(As Neo) (Mann-Whitney statistical comparison).

  • f P < 0.0005 against PC-3M-LN4 and PC-3M-LN4(As Neo) (χ2 test).