Table 2

Suppression of angiogenesis by dominant-negative IκBαM expression vector in human melanoma

A375SM control, A375SM-Neo, A375SM-IκM.1, A375SM-IκM.2, and A375SM-IκM.3 cells (1 × 106 cells/mouse) were injected s.c. into groups of nude mice (n = 5). Thirty-five days after tumor injection, the resulting tumors were removed and processed for immunohistochemical analysis. Vessels from these tumors were counted under a light microscope after immune staining of sections with anti-CD31 antibodies as stated in “Material and Methods.”

Cell lineMVD (× 102/mm2)
Mean ± SDMedian (range)Inhibition (%)
SM5.76 ± 0.516.02 (5.23–7.43)
Neo5.98 ± 0.725.41 (5.02–6.54)−3.8 ± 12.5
IκM.12.03 ± 0.35a2.12 (1.78–2.83)a64.8 ± 6.1a
IκM.21.56 ± 0.39a1.98 (1.42–2.32)a72.9 ± 6.8a
IκM.32.87 ± 0.46a2.56 (2.04–3.22)a50.2 ± 8.0a
  • a P < 0.01 as determined by Mann-Whitney U test.