Table 3

Sequences and properties of mutant phage isolated from heavy chain CDR3 library

Position9596979899100100A100B100CNo.IC50 (ng/ml)Kd (nm)Yield (%)
Original amino acidGYSSTaSYAM8.08.02.0
3rd round residuesSTa5b8.08.02.0
PSa4b8.0N.D.c17.5
PYa3b3.56.07.0
PNa2b4.511.010.0
PWa2b4.54.010.0
ADd2bN.D.N.D.N.D.
PIa1b4.55.010.0
WFa1b8.0N.D.10.0
PVa1b6.5N.D.7.0
Wa1b8.0N.D.5.0
4th round residuesSTa1e8.08.02.0
PNa3e4.511.010.0
PYa3e3.56.07.0
PFax2e6.020.03.0
PWa1e4.54.010.0
  • a Wild type.

  • b Number of clones of 22 clones.

  • c N.D., not determined.

  • d Could not make immunotoxin.

  • e Number of clones of 10 clones.