Table 4

Sequences and properties of mutant phage isolated from light chain CDR3 library

Position899091929394959697No.IC50 (ng/ml)Kd (nm)Yield (%)
Original amino acidLQSFNVPLT3.567
2nd round residuesSF4a3.567
SW6a1.337
SR3a6.098
SS2a15.02216
SL1a5.548
SM1a9.062
3rd round residuesSF1b3.567
SS7b15.02216
SW1b1.337
SR1b6.098
  • a Number of clones of 17 clones.

  • b Number of clones of 10 clones.