Table 1

Effect of NU1085 and NU1025 on TM-induced growth inhibition in human tumor cell lines

Cell lineIC50am)
TMTM + 10 μm NU1085TM + 50 μm NU1025TM + 200 μm NU1025
A549 (lung)949394 (2.4)b453 (2.1)301 (3.2)
1223466 (2.6)488 (2.5)373 (3.3)
CORL23 (lung)855446 (1.9)439 (1.9)427 (2.0)
984681 (1.4)977 (1.0)719 (1.4)
H522 (lung)>15001059 (>1.4)837 (>1.8)826 (>1.7)
1249705 (1.8)524 (2.4)645 (1.9)
SKOV3 (ovary)>1500639 (>2.3)842 (>1.8)427 (>3.5)
>1500867 (>1.7)810 (>1.9)339 (>4.4)
A2780 (ovary)525188 (2.8)231 (2.3)196 (2.7)
368183 (2.0)203 (1.8)188 (2.0)
OAW-42 (ovary)568419 (1.4)485 (1.2)364 (1.6)
714444 (1.6)649 (1.1)405 (1.8)
HT29 (colon)>1500881 (>1.7)733 (>2.0)415 (>3.6)
>1500719 (>2.1)623 (>2.4)429 (>3.5)
LoVo (colon)>1500828 (>1.8)813 (>1.8)474 (>3.2)
>1500821 (>1.8)572 (>2.6)277 (>5.4)
>1500929 (>1.6)961 (>1.6)611 (>2.5)
LS174T (colon)>1500432 (>3.5)749 (>2.0)421 (>3.6)
1371225 (6.1)363 (3.8)223 (6.1)
>1500766 (>2.0)1133 (>1.3)609 (>2.5)
MCF-7 (breast)>1500485 (>3.1)461 (>3.3)429 (>3.5)
>1500682 (>2.2)990 (>1.5)604 (>2.5)
T47D (breast)>1500747 (>2.0)956 (>1.6)834 (>1.8)
>15001547>15001150 (>1.3)
MDA-231 (breast)>15001211 (>1.2)1401 (>1.1)786 (>1.9)
14531152 (1.3)1378 (1.1)725 (2.0)
  • a IC50s were calculated from data from individual experiments, using five replicates for all samples.

  • b Numbers in parentheses, PF50s derived from the individual experiments. Where it was not possible to obtain IC50s for TM alone because of the limited solubility of this drug (i.e., IC50s were not achieved by the highest concentration (1500 μm) of TM used), PF50s were expressed as greater than the value calculated, assuming the IC50 for TM alone to be 1500μ m.