Table 4

Tumor cell detection reproducibility: ACIS-assisted analysis versus manual microscopy (specimens from breast cancer patients, n = 21)

SpecimenNo. of tumor cells
Initial analysisSecond review
Manual microscopyACIS-assisted analysisACIS manual analysisACIS-assisted analysis
10000
20222
30111
40222
50111
60444
70111
80222
90000
100121
110111
120333
130222
140000
150555
160111
170222
180000
190444
200222
210333
  • The number of tumor cells counted from a total of 21 bone marrow specimens from breast cancer patients that had initially been analyzed by both ACIS-assisted analysis and manual microscopy were reevaluated. For 17 specimens that had originally been classified as tumor cell negative by manual microscopy, a total of 37 objects were originally classified as tumor cells after ACIS-assisted analysis. These cells, along with 74 nontumor cells from the same 17 slides and five cells from each of four additional specimens (for a total of 131 cells) were recalled and reexamined by a pathologist, blinded to the original ACIS-assisted cellular classification. Finally, the pathologist reviewed ACIS montage images a second time, blinded to the original classification.