Antibodies and immunohistochemical procedure
| Antibody | Manufacturer | Clone | Citrate antigen retrieval (min) | Antibody dilution | Primary antibody incubation | Positive controls |
|---|---|---|---|---|---|---|
| MMP2 | Novocastra | 4D3 | 60 | 1:10 | 32 min at 41°C | Colon carcinoma |
| MMP9 | Novocastra | 2C3 | None | 1:15 | Overnight at 4°C | Colon carcinoma |
| TIMP1 | Neomarkers | 102D1 | None | 1:10 | 32 min at 41°C | Breast carcinoma |
| TIMP2 | Neomarkers | 2TMP04 | 60 | 1:10 | Overnight at 4°C | Breast carcinoma |