Table 1

Antibodies and immunohistochemical procedure

AntibodyManufacturerCloneCitrate antigen retrieval (min)Antibody dilutionPrimary antibody incubationPositive controls
MMP2Novocastra4D3601:1032 min at 41°CColon carcinoma
MMP9Novocastra2C3None1:15Overnight at 4°CColon carcinoma
TIMP1Neomarkers102D1None1:1032 min at 41°CBreast carcinoma
TIMP2Neomarkers2TMP04601:10Overnight at 4°CBreast carcinoma