Table 1

IL-8 induced stimulation of cell proliferation of human colon carcinoma cells with different metastatic potentials expressing different levels of IL-8

Caco2, KM12C, and KM12L4 cells expressing different levels of endogenous IL-8 were incubated with different concentrations of recombinant IL-8. The percentage of stimulation in cell proliferation by IL-8 was determined as described in “Materials and Methods.” The values are mean % stimulation ± SE. This is representative of three experiments done in triplicate.
IL-8 (ng/ml)% stimulation
Caco2KM12CKM12L4
0.000146.05 ± 13.8922.74 ± 5.3912.09 ± 3.86
0.00134.19 ± 15.68950.67 ± 5.3517.16 ± 3.92
0.0182.16 ± 16.73a40.95 ± 5.49a−0.99 ± 8.05
0.194.85 ± 19.21a55.49 ± 8.53a7.43 ± 7.12
1105.22 ± 27.473.55 ± 21.695.23 ± 5.42
1087.39 ± 16.0248.87 ± 6.87a26.48 ± 2.05
100100.98 ± 13.97a43.66 ± 9.43a7.82 ± 5.77
  • a Significantly different from KM12L4 cells expressing high levels of IL-8 at the corresponding concentrations of exogenously added recombinant IL-8 (P < 0.05).