Table 2

Assignment of peptide masses to the human RS/DJ-1 sequence

Analysis by MALDI-TOF mass spectrometry of the peptide masses followed by search in the NCBI database.
Measured mass (Da)Matched (Da)Delta (ppm)ResiduesSequence
Trypsin
875.8823875.9826114.504142–48DPVQCSR
1159.37991159.289078.371990 –98EILKEQENR
1547.06381546.7425207.729849 –62DVVICPDASLEDAK
1677.43431676.6554285.604513 –27GAEEMETVIPVDVMR
2283.21222282.7201215.5653100–122GLIAAICAGPTALLAHEIGFGSK
2410.92192410.894911.195699–122KGLIAAICAGPTALLAHEIGFGSK
2385.88402835.9015−7.32876–27ALVILAKGAEEMETVIPVDVMR
2586.10422585.883385.413864–89EGPYDVVVLPGGNLGAQNLSESAAVK
Chymotrypsin
983.4457983.1567293.912768 –77DVVVLPGGNL
1013.27041013.2734−3.00801–10ASKRALVILa
1072.03841072.3168−259.6541102 –112IAAICAGPTAL
1146.98281147.2344−219.3025142 –151SENRVEKDGL
1186.47841186.2706175.1733154 –164TSRGPGTSFEF
2942.12742942.5262−135.543411–38AKGAEEMETVIPVDVMRRAGIKVTVAGL
3235.23693234.6441183.278939–67AGKDPVQCSRDVVICPDASLEDAKKEGPY
Glu-C
760.9006760.828195.2907144 –149NRVEKD
1117.11931117.3111−171.665450 –59VVICPDASLE
1352.89361352.6664168.0011177 –189VAAQVKAPLVLKD
1375.46941375.6134−104.7112164–176FALAIVEALNGKE
1435.48791435.6256−95.9287150–163GLILTSRGPGTSFE
1567.57861567.7887−134.003769–84VVVLPGGNLGAQNLSE
1598.67011598.8685−124.0827117–131IGCGSKVTTHPLAKD
1598.84.021598.8900−31.13751–16ASKRALVILAKGAEEa
1678.67521678.8892−127.4453148–163KDGLILTSRGPGTSFE
2971.59112971.395565.8432117–143IGFGSKVTTHPLAKDKMMNGGHYTYSE
2972.29672971.8563171.222260–84DAKKEGPYDVVVLPGGNLGAQNLSE
  • a The peptide mass match is compatible with an Acet-N modification.