Table 2

Expression of MUC genes in pancreatic adenocarcinoma by RT-PCR analysis

Total RNA from 16 pancreatic tumor samples was isolated and subjected to semiquantitative RT-PCR as described in “Materials and Methods.” Amplification products were resolved on 1% agarose gel containing ethidium bromide. After exposure to UV light, the density of DNA bands was determined using the GelExpert software system (Nucleotech). Density values for MUC-specific amplification products were normalized against those for the internal control, GAPDH.

SampleClinical dataExpression levelc
Age (yr)SexD.S.aTNMbMUC1MUC2MUC3AMUC4MUC5ACMUC5BMUC6MUC7
184F3200+++c+++++
267M2100++++++
373F2210++++++
457M2210++++
574F3310++++++++++
674F2310+++++++
763M3310+++++
860F2210+++++++++++++
972F3300++++++
1050F2210++++++++
1143F2300++++++++++
1275F2310++++++++++
1373F1300++++
1464M1100+++++++++++
1577F4410++++++++
1662M3310+++++++
  • a D.S., differentiation stage.

  • b Tumor-Node-Metastasis classification.

  • c −, no expression; +, low level; ++, moderate level; +++, high level; ++++, very high level.