Table 3

Expression of MUC genes in chronic pancreatitis by RT-PCR analysis

Total RNA from 10 chronic pancreatitis samples was isolated and subjected to semiquantitative RT-PCR as described in “Materials and Methods.” Amplification products were resolved on 1% agarose gel containing ethidium bromide. After exposure to UV light, the density of DNA bands was determined using the GelExpert software system (Nucleotech). Density values for MUC-specific amplification products were normalized against those for the internal control, GAPDH.

SampleExpression levela
MUC1MUC2MUC3AMUC4MUC5ACMUC5BMUC6MUC7
1+a++++++
2++++++
3+++
4+++++++++
5+++++
6++++
7++++++++
8++++++
9++++++
10+++++
  • a −, no expression; +, low level; ++, moderate level; +++, high level; ++++, very high level.