The expression of MAGE genes and HLA typing in established bladder cancer cell linesa,b
| Cell line | Pathology | MAGE-1 | MAGE-2 | MAGE-3 | HLA-A2 | HLA-A24 |
|---|---|---|---|---|---|---|
| KU-1 | TCC, G2 | − | − | − | − | − |
| KU-7 | TCC, G1 | ++ | ± | +++ | + | − |
| KU-19-19 | TCC, G3 | − | + | +++ | + | − |
| T24 | TCC, G2 | − | + | ± | + | + |
| FY | TCC, G3 | ++ | +++ | +++ | − | + |
a Expression of MAGE genes was detected by RT-PCR amplification of total RNA isolated from cell lysates of each cell line using specific oligonucleotide primers.
b The relative levels of gene expression were determined as follows: the strongest positive band was defined as 100% and graded “−” for no expression; ±, <2% positive; +, 2–25% positive; ++, 26–50% positive; +++, >50% positive.