Table 1

Expression of MUC genes in pancreatic tumor cell lines by RT-PCR analysis

Total RNA from 15 pancreatic tumor cell lines was isolated and subjected to semiquantitative RT-PCR as described in “Materials and Methods.” Amplification products were resolved on 1% agarose gel containing ethidium bromide. After exposure to UV light, the density of DNA bands was determined using the GelExpert software system (Nucleotech). Density values for MUC-specific amplification products were normalized against those for the internal control, GAPDH.

Cell lines	D.S.^a	Expression level^b
Cell lines	D.S.^a			MUC1	MUC2	MUC3A	MUC4	MUC5AC	MUC5B	MUC6	MUC7
SC2P9	WD	+	++	−	+++	++	−	−	−
Capan 1	WD	+++	+++	−	++++	+++	+++	+++	−
Capan 2	WD	++	−	−	++++	−	−	−	−
HPAF	WD	+++	+	−	++++	+++	++	−	−
Panc1	PD	+	−	−	−	++	+++	−	−
ASPC-1	ND	+++	−	−	++	−	+++	−	−
HCG25	PD	+++	−	−	−	−	−	−	−
T3M4	MD	++	+++	−	+++	−	+	−	−
Colo357	WD	++++	−	−	+++	++	+++	−	−
BxPC3	MD	+++	++	−	+++	−	+	+++	−
MiaPaCa	ND	+	−	−	−	−	−	−	−
HPAC	ND	++++	−	−	+++	++	−	−	−
QGP1	ND	+	−	−	+++	−	−	−	−
HS766T	ND	+	+	−	−	+++	++	++	−
Panc89	MD	ND	++	−	+++	++	++	−	−
Pancreas^c		+++	−	−	−	+++	+++	++++	−
Trachea^c		++	++	−	+++	++++	++++	−	−
S.I.^c		+	++++	++++	+	−	−	++++	−
S.G.^c		ND	−	−	+++	−	++	−	++++

^a D.S., differentiation stage; PD, poorly differentiated; MD, moderately differentiated; WD, well differentiated; ND, not determined.
^b −, no expression; +, low level; ++, moderate level; +++, high level; ++++, very high level; ND, not determined.
^c Normal tissue samples were included as controls. S.I., small intestine; S.G., salivary gland.