Table 1

Expression of MUC genes in pancreatic tumor cell lines by RT-PCR analysis

Total RNA from 15 pancreatic tumor cell lines was isolated and subjected to semiquantitative RT-PCR as described in “Materials and Methods.” Amplification products were resolved on 1% agarose gel containing ethidium bromide. After exposure to UV light, the density of DNA bands was determined using the GelExpert software system (Nucleotech). Density values for MUC-specific amplification products were normalized against those for the internal control, GAPDH.

Cell linesD.S.aExpression levelb
MUC1MUC2MUC3AMUC4MUC5ACMUC5BMUC6MUC7
SC2P9WD++++++++
Capan 1WD+++++++++++++++++++
Capan 2WD++++++
HPAFWD+++++++++++++
Panc1PD++++++
ASPC-1ND++++++++
HCG25PD+++
T3M4MD+++++++++
Colo357WD++++++++++++
BxPC3MD++++++++++++
MiaPaCaND+
HPACND+++++++++
QGP1ND++++
HS766TND+++++++++
Panc89MDND+++++++++
Pancreasc+++++++++++++
Tracheac+++++++++++++++
S.I.c++++++++++++++
S.G.cND+++++++++
  • a D.S., differentiation stage; PD, poorly differentiated; MD, moderately differentiated; WD, well differentiated; ND, not determined.

  • b −, no expression; +, low level; ++, moderate level; +++, high level; ++++, very high level; ND, not determined.

  • c Normal tissue samples were included as controls. S.I., small intestine; S.G., salivary gland.