Table 1

Oligonucleotides used for genomic PCR of exon 14 of the DPYD gene

PrimerSequenceDirectionPositiona
DPD14f5′-TCCTCTGCAAAAATGTGAGAAGGGACC-3′Sense451–477
DPD14r5′-TCACCAACTTATGCCAATTCTC-3′Antisense762–783
NDEf5′-ATCAGGACATTGTGACATATGTTTC-3′bSense565–589
NDEr5′-CTTGTTTTAGATGTTAAATCACACATA-3′bAntisense736–762
  • a Numbering according to the intron sequences flanking exon 14 of DPYD as published by Vreken et al. (19) .

  • b The NdeI restriction sites introduced by site-directed mutagenesis (primers NDEf and NDEr) are underlined. The single-base mismatches introducing the Nde1 restriction site are depicted in boldface.