Table 1

EGFR, TGF-α, bFGF, and VEGF expression in human cancer cell lines

EGFR expression was determined by Western blotting. EGFR relative levels were determined as compared with EGFR levels in GEO cells. GEO cells possess ∼40,000 binding sites/cell as determined by 125I-labeled EGF binding assay (23) . TGF-α, bFGF, and VEGF secretion was determined on 24-h-collected serum-free CM by using specific ELISA kits, as described in “Materials and Methods.” Results were normalized for the number of producing cells and reported as pg of growth factor/106 cells/24 h.
Cell lineEGFR (relative expression)TGF-α (pg/106 cells/24 h)bFGF (pg/106 cells/24 h)VEGF (pg/106 cells/24 h)
ZR-75-10.6285 ± 30195 (±10)85 ± 5
MCF-7 ADR6.2580 ± 25255 ± 3575 ± 5
OVCAR-33.7680 ± 35645 ± 5555 ± 4
GEO1405 ± 15710 ± 55150 ± 8
SW4803.5480 ± 20600 ± 65400 ± 12
CaCo20.6255 ± 15NDa110 ± 8
KATO III1340 ± 25ND180 (±8)
N870.4360 ± 35380 ± 40145 (±20)
  • a ND, not detectable.