Table 3

Effects on colony formation in soft agar of TC-71 cells by combination treatmenta

IMDM 10% FCSMOPC-21αIR3
No. of colonies ± SEInhibition %bNo. of colonies ± SEInhibition %bNo. of colonies ± SEInhibition %b
Control1019 ± 46963 ± 5913.5328 ± 4267.8
DXR, 3 ng/ml977 ± 1374.1816 ± 3726.7144 ± 6c85.9
DXR, 10 ng/ml888 ± 3612.8722 ± 2535.279 ± 6c92.2
DXR, 30 ng/ml711 ± 1830.2623 ± 1644.049 ± 6d95.2
DXR, 100 ng/ml249 ± 3775.5231 ± 3479.36 ± 1c99.5
VCR, 300 pg/ml875 ± 2414.1NDeND370 ± 33c63.7
VCR, 600 pg/ml744 ± 8227.0NDND293 ± 8c71.2
VCR, 1 ng/ml290 ± 2571.5NDND96 ± 7c90.5
  • a Cells (3300) were plated in 0.33% agarose with medium plus 10% FCS in the absence (control) or in the presence of increasing concentrations of doxorubicin (DXR) or vincristine (VCR) ± αIR3 (1 μg/ml), and colonies were counted after 7 days. Data are expressed as mean of triplicate plates ± SE.

  • b Percentages were calculated with respect to control.

  • c P < 0.05; Student’s t test, compared with the corresponding dose of DXR, or VCR.

  • d P < 0.001; Student’s t test, compared with the corresponding dose of DXR, or VCR.

  • e ND, not determined.