Table 1

Assessment of cell cycle by fluorescent microscopy of SKBr-3 cells treated with DMSO, 10 nm Taxol for 4 h, 10 nm Taxol followed by 50 nm 17-AAG, or 24-h pretreatment with 50 nm 17-AAG followed by 10 nm Taxol

Cells were fixed with paraformaldehyde and stained with a fluorescent-labeled anti-β-tubulin and with Hoechst 33258 for nuclear DNA.
DrugInterphaseMitosisApoptosis
Untreated93 ± 0.75 ± 0.72 ± 0.4
AAG 50 nm92 ± 0.83 ± 0.65 ± 0.3
Paclitaxel 10 nm76 ± 1.017 ± 1.07 ± 0.3
Paclitaxel → AAG65 ± 1.213 ± 1.422 ± 1.0
AAG → Paclitaxel89 ± 1.05 ± 0.86 ± 0.9