Table 2

Summary data of genes tested

GeneGene locationForward primerReverse primerTemperature (°C)Cycle no.Reference no.
FHIT 3p14M: 5′-TTGGGGCGCGGGTTTGGGTTTTTACGC-3′aM: 5′-CGTAAACGACGCCGACCCCACTA-3′71–6440 33
U: 5′-TTGGGGTGTGGGTTTGGGTTTTTATG-3′U: 5′-CATAAACAACACCAACCCCACTA-3′71–6440
RASSF1A 3p21M: 5′-GGGTTTTGCGAGAGCGCG-3′M: 5′-GCTAACAAACGCGAACCG-3′6440 17
U: 5′-GGTTTTGTGAGAGTGTGTTTAG-3′U: 5′-CACTAACAAACACAAACCAAAC-3′5940
RARβ 3p24M: 5′-TCGAGAACGCGAGCGATTCG-3′M: 5′-GACCAATCCAACCGAAACGA-3′6235 16
U: 5′-TTGAGAATGTGAGTGATTTGA-3′U: 5′-AACCAATCCAACCAAAACAA-3′6235
APC 5q21M: 5′-TATTGCGGAGTGCGGGTC-3′M: 5′-TCGACGAACTCCCGACGA-3′6435 34
U: 5′-GTGTTTTATTGTGGAGTGTGGGTT-3′U: 5′-CCAATCAACAAACTCCCAACAA-3′6235
p16 INK4A 9p21M: 5′-TTATTAGAGGGTGGGGCGGATCGC-3′M: 5′-GACCCCGAACCGCGACCGTAA-3′6535 6
U: 5′-TTATTAGAGGGTGGGGTGGATTGT-3′U: 5′-CAACCCCAAACCACAACCATAA-3′6035
DAPK 9q34M: 5′-GGATAGTCGGATCGAGTTAACGTC-3′M: 5′-CCCTCCCAAACGCCGA-3′6435 35
U: 5′-GGAGGATAGTTGGATTGAGTTAATGTT-3′U: 5′-CAAATCCCTCCCAAACACCAA-3′6435
MGMT 10q26M: 5′-TTTCGACGTTCGTAGGTTTTCGC-3′M: 5′-GCACTCTTCCGAAAACGAAACG-3′6635 36
U: 5′-TTTGTGTTTTGATGTTTGTAGGTTTTTGT-3′U: 5′-AACTCCACACTCTTCCAAAAACAAAACA-3′6635
GSTP1 11q13M: 5′-TTCGGGGTGTAGCGGTCGTC-3′M: 5′-GCCCCAATACTAAATCACGACG-3′6437 9
U: 5′-GATGTTTGGGGTGTAGTGGTTGTT-3′U: 5′-CCACCCCAATACTAAATCACAACA-3′6437
CDH1 16q22M: 5′-GGTGAATTTTTAGTTAATTAGCGGTAC-3′M: 5′-CATAACTAACCGAAAACGCCG-3′5737 37
U: 5′-GGTAGGTGAATTTTTAGTTAATTAGTGGTA-3′U: 5′-ACCCATAACTAACCAAAAACACCA-3′5737
CDH13 16q24M: 5′-TCGCGGGGTTCGTTTTTCGC-3′M: 5′-GACGTTTTCATTCATACACGCG-3′6437 22
U: 5′-CGCGGGGCTCGCTCCTCGC-3′U: 5′-GGCGTTTTCATTCATGCACGCG-3′6437
  • a M, methylated-specific primers; U, unmethylated-specific primers.