Sequences of mutant phage obtained after panning
Amino acid sequences of mutant phage isolated after two rounds of panning are listed. The entire Fv of each phage was sequenced. Only amino acids in the target region are shown. Each residue is numbered according to the Kabat database (21) .
| 99 | 100 | 100A | 100B |
|---|---|---|---|
| G | S | S | Ya |
| G | T | H | Wb |
| G | Y | N | Wb |
| G | T | T | Wb,c |
| G | S | T | Yb |
| G | K | N | Rb,c |
| G | S | T | Rd |
| G | H | T | F |
| G | N | R | Y |
| G | T | A | Y |
| G | T | N | Y |
| G | L | H | Y |
| G | F | L | Y |
| G | S | R | Y |
| G | R | N | Y |
| G | V | H | R |
| G | A | L | R |
| G | V | R | A |
| G | T | A | K |
| G | R | T | S |
a WT.
b Immunotoxin made.
c Clone found three times.
d Clone found two times.