Summary of BIAcore analysis mutants of RFB4-PE38
| Binding kinetics were analyzed on a BIAcore 2000 Biosensor (BIAcore). On and off rates were measured by injecting immunotoxins at a concentration of 380 nm over a CM5 sensor chip (BIAcore) containing immobilized CD22. Bound material was allowed to dissociate for 5 min or more. | ||||||
|---|---|---|---|---|---|---|
| Immunotoxin | Kon (M−1S−1) | Koff (S−1) | Kda (nm) | |||
| GSSY (WT) | 2.08 × 104 | 1.77 × 10−3 | 85 | |||
| GTHW | 3.27 × 104 | 2.07 × 10−4 | 6 | |||
| GYNW | 1.84 × 104 | 1.91 × 10−4 | 10 | |||
| GTTW | 2.62 × 104 | 6.5 × 10−4 | 24 | |||
| GSTY | 3.15 × 104 | 1.55 × 10−3 | 49 | |||
a Kd was calculated by dividing Koff by Kon.