Table 1

Quantitation of RhoB expression in head and neck squamous cell carcinoma

The Optimas 6.5 Image Analysis software was used to quantitate nuclear RhoB expression in 18 squamous cell carcinomas containing superficial invasive component (SIC) and deeply invasive component (DIC) areas of the tumor, as well as in adjacent areas of carcinoma in situ (CIS) and nonneoplastic squamous epithelium. RhoB quantitation was also performed independently in well-differentiated (WD), moderately-differentiated (MD), and poorly-differentiated (PD) areas of the tumors. The intensity of RhoB staining is expressed as a RhoB index. The average and SD for each of the grouped areas are depicted at the bottom of the table. A Wilcoxon Rank-Sum test analysis showed significant differences (P < 0.001) between nonneoplastic epithelium, SIC, and DIC, between CIS, SIC, and DIC, and between SIC and DIC. No significant differences were found between nonneoplastic epithelium and CIS. Significant differences (P < 0.0001) were also observed among the three degrees of differentiation.

CaseSexAge (yrs)LocationSize (cm)TNMStageFollow-up (mon)StatusNonneoplasticCISSICDICWDMDPD
1M61Pyriform sinus2.0T2N0M0II14NEDa969516432155
2F67Oral cavity2.6T2N0M0II73AED8087722643
3F76Oral cavity4.0T2N0M0II18AED69311332386
4F62Larynx3.1T2N0M0II45AED70425044665
5M70Oral cavity2.5T2N0M0II35NED927647363454
6F52Oral cavity3.5T2N1M0III19NED7230270.52931
7M77Larynx2.0T3N0M0III21NED77682222733
8M77Larynx3.7T3N0M0III35NED87521912341
9M42Eyelid4.2T4N0M0IV14NED92901221853
10M63Larynx4.1T4N0M0IV16NED928832128163
11M56Tongue6.6T4N1M0IV36DOD73624316482
12F82Tongue5.5T4N1M0IV26AED82691713471
13M54Oropharynx4.2T4N1M0IV46DOD797338323243
14M57Larynx3.1T3N2BM0IV38AED777043113184
15M69Tongue6.0T3N2BM0IV12NED605257449152
16M71Pyriform sinus8.0T4N2BM0IV14AED66671811090.5
17F69Larynx4.5T4N2CM0IV10NED587329321156
18M69Lip3.5T4N2CM0IV12NED927557247105
Average78.566.530.12.232123.2
SD11.59.016.01.115.810.41.7
  • a NED, no evidence of disease; AED, alive with evidence of disease; DOD, dead of disease.