Table 1

Primer sequences and PCR conditions

PrimersSequencesB95-8 coordinatesProduct size (bp)PCR condition
For fresh frozen tumor tissue
 4P5′-TCCGGGCTGCGAGTAATTGG-3′107754–10777394°C for 5 min: 1 cycle
 4M5′-GGACACCATCTCTATGTCTTGGCC-3′108137–10816040794°C for 30 s, 58°C for 30 s, 72°C for 60 s: 30 cycles
72°C for 5 min; final extension
 4P15′-GGGCCAAGACATAGAGATGGTGTCC-3′108136–10816095°C for 9 min 1 cycle
 4P2R5′-GCGGTGGAGACCCGGATGATGATGA-3′109111–10913522694°C for 30 s, 60°C for 60 s: 43 cycles
60°C for 10 min; final extension
 4P25′-TCATCATCATCCGGGTCTCCACCGC-3′109111–10913594°C for 5 min: 1 cycle
 4M15′-CCCAAGTTCCTTCGTCGGTAGTCC-3′109436–10945934994°C for 30 s, 61°C for 30 s, 72°C for 60 s: 30 cycles
72°C for 5 min; final extension
 4P35′-AGATGACCCAGGAGAAGGCCCAAGC-3′109266–10929094°C for 5 min: 1 cycle
 4M25′-GGCCAGAAATACACCAACAGCACGC-3′109960–10998471994°C for 30 s, 64°C for 30 s, 72°C for 60 s: 30 cycles
72°C for 5 min; final extension
For peripheral-blood cells
 Outer5′-GTAGAAGGCCATTTTTCCAC-3′109151–10917094°C for 5 min: 1 cycle
5′-CTCCATCGTCAAAGCTGCA-3′109741–10975960994°C for 30 s, 58°C for 30 s, 72°C for 40 s: 25 cycles
72°C for 10 min; final extension
 Inner5′-AGATGACCCAGGAGAAGGCCCAAGC-3′109266–10929094°C for 5 min: 1 cycle
5′-CAAAGGGGAGACGACTCAATGGTGT-3′109549–10957330894°C for 30 s, 58°C for 30 s, 72°C for 40 s: 25 cycles
72°C for 10 min; final extension