Table 1

Primers and probes

Primers and probe sequences used in the real-time QPCR experiments. Two primer-probe pairs were designed, one for the target gene ARF exon 1β and the other for the reference gene GAPDH.

Primer/probeSequence 5′ > 3′ReporterQuencher
ARF exon 1β senseGGA GGC GGC GAG AAC AT
ARF exon 1β antisenseTGA ACC ACG AAA ACC CTC ACT
ARF probeTGC GCA GGT TCT TGG TGA CCC TCCVICTAMRAa
GAPDH senseCCA CTA GGC GCT CAC TGT TCT
GAPDH antisenseGCG AAC TCA CCC GTT GAC T
GAPDH probeCTC CCT CCG CGC AGC CGA GCFAMTAMRA
  • a TAMRA, 6-carboxytetramethylrhodamine.