Table 2

Cell lines with homozygous deletions, analyzed with QPCR

Results from deletion and duplication analysis in the cell lines: IGR-1, SK-MEL-5, and WM-266-4. Cell lines were contaminated with normal DNA from 0–90% for IGR-1 and from 0–50% for SK-MEL-5 and WM-266-4; the template amount was constant. Only normal DNA was used for 100% contamination. Test parameter was amount of ARF exon 1β/GAPDH. Samples were run in duplicates (1 and 2) and average values were calculated (X).

Wild type DNA contaminationIGR-1SK-MEL-5WM-266-4
12X12X12X
0%0.000.000.000.000.000.000.000.000.00
10%0.130.120.130.100.100.100.090.100.09
20%0.230.230.230.220.170.200.180.140.16
30%0.290.320.310.250.300.270.270.260.26
40%0.460.440.450.400.400.400.380.380.38
50%0.510.510.510.410.480.440.450.440.45
60%0.600.630.61
70%0.680.680.68
80%0.820.830.83
90%0.830.880.85
100%1.061.071.06