Table 3

Real-time QPCR results compared to results from the D9S942 marker

Results from real-time QPCR were compared with informative cases from the microsatellite marker D9S942.

Real-time QPCRD9S942 informativeD9S942 not informativeTotal
HETLOHHomozygoteNot Analyzeda
HD1663126
LOH7 11 2222
LOH/WT 5 2 029
WT 54 9131389
WT/DUP 15 1 3120
DUP × 1b 11 0 3317
DUP >1 1 1 013
Total109302423186
Matchingc701181
  • a Not analysed includes 6 borderline cases LOH/heterozygote WT, for the D9S942 marker.

  • b DUP × 1: possible one copy amplification, DUP >1 more than one copy amplification.

  • c QPCR borderline cases (LOH/WT and WT/DUP) and possible duplications were excluded from calculations (italic numbers). Bold numbers are matching cases, in total 81 (79%), and underlined numbers are nonmatching cases, in total 22 (21%).