Table 3

The effect of treatment with cytotoxic LH-RH analogue AN-152 and DOX on mRNA expression of EGFR, HER-2 and -3, and the binding characteristics of LH-RH-receptors on s.c. implanted MDA-PCa-2b tumors in nude mice

mRNA % of controlLH-RH-receptor-binding characteristicsa
Treatment groupsEGFRHER-2HER-3Kd (nm)Bmax (fmol/mg protein)
1. Control100 ± 5.8100 ± 2.2100 ± 3.46.2 ± 0.7657.8 ± 24.6
2. Decapeptyl65.8 ± 2.5b86.4 ± 6.587.2 ± 6.65.9 ± 0.4565.9 ± 41.6
3. DOX85.6 ± 5.0b86.3 ± 5.161.4 ± 4.5b6.5 ± 0.4647.1 ± 13.5
4. AN-15274.3 ± 2.3b81.9 ± 3.5b65.8 ± 10.3b5.1 ± 0.6419.5 ± 9.3b
  • a Binding characteristics were obtained from 12-point displacement experiments. All values represent mean ± SE of three independent experiments, each done in duplicate or triplicate.

  • b P < 0.01 vs. control.