Table 1

Hydrolase activities and CES2 band densities of 24 colon tumors

Colon tumors 1–18 are primary and 19–24 are metastatic tumors. The 4-methylumbelliferyl acetate hydrolase activity was determined in 50 mm sodium phosphate buffer (pH 7.4) at 37°C. Concentration of 4-methylumbelliferyl acetate was set at 0.5 mm, and specific activity is described as μmol mg−1 h−1. CPT-11 hydrolase activity was determined in 50 mm HEPES buffer (pH 7.4), with 10% ethylene glycol in a volume of 250 μl for 24 h at 37°C, and CPT-11 concentration was set at 50 μm, and the specific activity is reported in pmol mg−1 h−1. The CES2 band densities for carboxylesterase activities on nondenaturing gel were in fluorescence units.

Tumor no.CPT-11 hydrolase activity pmol mg−1 h−14-MUA activity, μmol mg−1 h−1CES2 band density
15.02.88913
22.53.771789
31.72.811158
41.12.28740
54.35.283817
612.510.083614
72.63.66888
81.22.39721
98.12.44785
103.42.531234
1138.28.218721
1245.18.756347
134.431871
143.01.09244
150.81.56232
162.83.241268
174.66.062478
182.03.3926
192.60.36340
204.21.691827
219.01.81586
2223.15.555377
236.82.22764
248.12.38803