Table 1

Cytotoxic effect of full-length endostatin and their fragments on different cell lines

CompoundCytotoxicity (% of dead cells/total)a
CVECMAEBASMNIH-3T3A431
None25 ± 815 ± 26 ± 18 ± 310 ± 3
mEnd25 ± 713 ± 63 ± 28 ± 28 ± 2
Fragment III19 ± 516 ± 54 ± 19 ± 35 ± 4
Fragment IV27 ± 914 ± 27 ± 25 ± 16 ± 2
Fragment IVox24 ± 718 ± 46 ± 27 ± 29 ± 3
  • a Incubation of cell lines (CVECs, MAEs, BASMs, murine fibroblasts NIH-3T3, and HS epidermoid A431 cells) was carried out for 4 h at 37°C with 300 ng/ml of the compounds. Data are reported as percentage of dead cells over the total number of cells (n = 5 for CVECs and n = 2 experiments for the other cells, run in quadruplicate).